热断层成像技术用于乳腺癌裸鼠模型实验初探

Primary evaluation in nude mouse model with human breast cancer by thermal texture maps

目的探讨热断层成像技术(TTM)应用于乳腺癌裸鼠模型扫描的可行性。方法采用既往实验中用逆转录病毒介导RNA干扰的方法,已构建的能不同程度抑制人的乳腺癌细胞MCF7中LRP16基因表达的两株细胞pL374/MCF7(抑制率90%)和对照细胞系pGFPi/MCF7(阴性);选取BALB/c雌性裸小鼠共20只,随机分为两组,10只/组。分别接种pL374/MCF7及对照细胞系pLGFPi/MCF7。接种细胞6周后,用TTM观察裸鼠成瘤情况。将20只裸鼠处死,解剖观察,取形成的小结节和相应的肺组织进行HE染色。结果TTM值异常升高的裸鼠有13只。11只裸鼠有≤3mm小结节。HE结果显示,以上11只裸鼠体内形成的小结节为阳性肿瘤组织,4只肺部组织显示有阳性肿瘤细胞。TTM值异常升高与HE染色阳性高度相关。结论TTM技术可能在肿瘤的动物模型研究中发挥一定的作用。

Objective To explore the diagnostic feasibility of thermal texture maps （TTM） for breast cancer in sub-clinical stage by using nude mouse model. Methods Two MCF-7 human breast cancer cell lines with different LRP16 expression level by retrovirus-mediated siRNA strategy, pL374/MCF-7 （suppressive rate 90%） and pGFPi/MCF-7 （negative control） were used as model cells. Twenty BALB/C female nude mice were divided into two groups randomly. Cells were inoculated subcutaneously into the nude mice. Six weeks later, nude mice were examined by TTM, then were killed and anatomized. Mini-nodule and lung tissue were dissected for HE staining. Results The relative heat radiation intensity in 13 mice was higher than others. Whereas, 11 of 13 cases have less than 3 mm mini-nodule. By HE staining, the results show that 11 cases positive in mini-nodule and 4 cases positive in lung tissue. There was high correlation between TTM and HE staining. Conclusion Our results indicated that TTM technology might provide powerful screening for breast cancer in early stage, which can only be diagnosed by pathological analysis. In addition, TTM can be a useful tool for tumor-carried mouse research. T