牡丹皮多糖对免疫抑制小鼠的免疫调节作用

Immunoregulatory Effects of Polysaccharide of Mudan Cortex on Immunosupressive Mice

目的研究牡丹皮多糖对免疫功能低下小鼠的免疫调节作用。方法采用药物、荷瘤的方法建立免疫抑制小鼠动物模型,利用MTT方法淋巴细胞转化及巨噬细胞吞噬中性红实验,检测牡丹皮多糖对免疫抑制小鼠免疫反应的影响。结果牡丹皮多糖可增强吞噬细胞的吞噬功能,增加抗体形成细胞的数量,促进淋巴细胞转化。结论牡丹皮多糖具有增强小鼠免疫功能的作用。

Objective To study immunoregulatory activity of polysaccharide of mudan cortex （PSM） on immunodepressive mice. Method The immunodepressive mice model was established with cyclophosphamide treated and tumor-bearing. PSM was orally administered to mice for 10 d. MTT method was used to observe the prolifernation of lymphocytes. The function of macrophage in abdominal activity was measured. Sectrophotometric determination was adopted to evaluate the ability of plaque forming cell. Result PSM could enhance the function of macrophage, increase the content of plaque forming cell, and promote the prelifernation of lymphocytes. Conclusion PSM could enhance immunofunction of immunodepressive mice.