栀子总苷的抗炎镇痛作用研究

Anti-inflammatory and Analgesic Effects of Total Glucosides of Cape Jasmine

目的研究栀子总苷(TGCJ)的抗炎镇痛作用。方法采用角叉菜致大鼠足肿胀模型、醋酸致小鼠腹腔毛细血管通透性增加模型、棉球致肉芽组织增生模型,观察TGCJ的抗炎作用;采用热板法测定小鼠的痛阈、醋酸诱发的小鼠扭体反应,观察TGCJ的镇痛作用。结果TGCJ的80、40mg/kg2个剂量组ig给药,对角叉菜致大鼠足肿胀、棉球致肉芽组织增生均有明显抑制作用,TGCJ的160、80、40mg/kg3个剂量组ig给药,对醋酸致小鼠腹腔毛细血管通透性增加有抑制作用,且抑制作用呈剂量相关趋势;TGCJ的160、80、40mg/kg3个剂量组ig给药,可以明显升高小鼠的痛阈,对醋酸诱发的小鼠扭体反应有一定抑制作用。结论TGCJ有明显的抗炎和一定的镇痛作用,确定TGCJ为栀子抗炎镇痛的有效部位。

Objective To research anti-inflammatory and analgesic effects of total glucosides of Cape Jasmine （TGCJ）. Method Claw swelling induced by carrageen, capillary permeability increase induced by acetic acid and tampon granuloma induced with tampon embedding method were studied to observe the anti-inflammatory effects of TGCJ. Pain threshold of mice were determined with hot-plate test and the response of writhes induced by acetic acid to observe the analgesic effects of TGCJ. Result The large, medium dosage of TGCJ （80, 40 mL/kg） significantly inhibited carrageen-induced rat paw edema and tampon granuloma. The large, medium, small dosage of TGCJ （160, 80, 40 mL/kg） significantly inhibited the capillary permeability induced by acetic acid. The large, medium , small dosage of TGCJ （160, 80, 40 mL/kg） reduced the number of writhes induced by acetic acid in mice, and increased pain threshold of mice. Conclusion TGCJ has significant anti-inflammatory and analgesic effects, and TGCJ is the effective part obtained from Cape Jasmine.