肾上腺素α2受体在豚鼠血管纹的定位定量研究

The Quantification and Localization Studies of α 2 Adrenoceptonin the Stria Vascularis of Guinea Pig

用竞争蛋白结合分析法及放射自显影法对豚鼠血管纹肾上腺素α２受体进行了定性定位定量测定，结果显示：加入去甲肾上腺素组（Ａ组）中ｃＡＭＰ量仅为１．３８９±０．８５２ｐｍｏｌ／４０μｌ，较对照组（Ｃ组）７．７００８±０．８６７ｐｍｏｌ／４０μ显著低（Ｐ＜０．０５）；先加入育亨宾再加去甲肾上腺素组（Ｂ组）中ｃＡＭＰ量又复回升达６．９５４±０．９６２（Ｐ＜０．０５），但仍较对照组低（Ｐ＜０．０５）。在经３Ｈ－育亨宾孵育的切片中，血管纹中下部银粒数为９６±１４个／视野，呈条带状分布，与血管纹走向一致，血管纹之外的组织银粒散在均匀，银粒数为２８±７／视野。经细胞立体计量法计算，血管纹中α２受体的含量大约为１．２５×１０９个／ｍｍ３。作者认为，豚鼠血管纹边缘细胞存在α２受体，并推想该受体是通过膜受体－ｃＡＭＰ途径影响细胞膜上Ｎａ＋－Ｋ＋－ＡＴＰ酶的活性，从而参与内淋巴的平衡。

With CPBA and ARG,the qualitative and quantitative analyses,and also the localization of α 2 adrenoceptors were conducted in the stria vascularis in guinea pig.CPBA studies show that the content of cAMP in group A was 1.389±0.852 pmol/40μl,in group C 7.7008±0.867 pmol/40μl,in group B 6.958±0.962.ARG studies show that the grains were concentrated in the stria vascularis,especially in middle and basal parts(96±14 grains/microfield) and in the otherthinner parts (28±7 grains/microfield).The quantity of α 2 adrenoceptor in stria vascularis were about 1.25×10 9 reseptors/mm 3 by cell morphometry.In conclusion,the α 2 adrenoceptor presented in the stria vascularis.The possible machanism of modulating endolmph was to affect the activity of Na + K + ATPase by membrane receptor cAMP.