慢性乙型肝炎患者血清SIL－2R及外周血T淋巴细胞亚群检测

THE MEASUREMENT OF SERUM SIL-2R AND T CELL SUBSETS OF PBMC IN CHRONIC HEPATITIS B

应用双抗体夹心法检测了５４例慢性乙型肝炎、肝硬化患者血清可溶性白介素２受体（ＳＩＬ－２Ｒ），应用单克隆抗体和ＡＰＡＡＰ法对Ｔ淋巴细胞亚群进行了检测，结果示慢性乙型肝炎病人血清ＳＩＬ－２Ｒ明显高于对照组，并且其升高与血清胆红素水平相关，而病人ＯＫＴ３细胞与正常对照组无显著差异，ＯＫＴ４阳性细胞比例下降，ＯＫＴ８阳性细胞比例增加，ＯＫＴ／ＯＫＴ比值下降，各临床类型中以重症肝炎ＯＫＴ／ＯＫＴ比值下降显著，其次为慢活肝，说明随着肝细胞坏死程度加重，免疫紊乱状态更加严重。

Soluble interleukin-2 receptor in the sera of 54 patients with chronic hepatitis B were quantified by Sandwich ELISA with monoclonal and polyclonal antibodies.In addition,the changes of T cell subsets in peripheral blood of the patients were observed.The result showed that the patients with chronic hepatitis B had high serum SIL-2R concentrations which corelated with bilirubin level of serum.As compared with the control group,OKT3 cell was of no difference,OKY4/OKT8 ratio decreased significantly in these patients.This resulted from decrease in OKT4 positive cells and increase in OKT8 positive cells.The decrease of OKT4/OKT8 ratio was even more significant in chronic serious hepatitis than in other clinical class.Secondly the decrease of the ratio was chronic activity hepatitis.We can conclude that the more is serious necrosis is,the more serious immunodisorder.