西葫芦组培苗驯化和移栽影响因素分析

Analysis of main factors affecting the acclimatizing and transplanting of regenerated plantlets of summer squash(Cucurbita pepo L.)in vitro culture

以西葫芦离体雌核发育而成的胚囊植株组培苗为试材,分析影响西葫芦组培苗驯化、移栽成活率的若干因素。结果表明,开始驯化时组培苗的苗龄、驯化温度、移栽基质组成、驯化方式等对西葫芦组培苗的驯化、移栽有较大影响;7～8叶1心的组培苗,在昼温22～28℃、夜温12～18℃的变温条件下,先闭瓶适应自然光照再开瓶逐步适应自然湿度,采用蛭石∶草炭=1∶1的移栽基质配方,成活率在95.0%以上。

In this research the regenerated embryonary sac plants was used for studying the main factors which effected the acclimatizing and transplanting of regenerated plantlets of summer squash （Cucurbita pepo L. ）in vitro. The results indicated that the age of the plantlet,the acclimatizing temperature,the compositions of the culture nutrient soil and the acclimatizing procedures were the main factors of the transplanting survival rates. The good conditions of acclimatizing and transplanting for the regenerated plantlet was 7-8 leaves stage, cultured at 22-28℃ day and 12-18℃ night,first adapted to the sunlight and then to the humidity out of the culturing bottles,planted in vermiculite ： straw charcoal = 1 ： 1 ,and the tansplanting survival rates of the regenerated plants were above 95.0%.