摘要
为探讨白细胞介素及白细胞介素受体在选择性IgA缺乏症发病过程中的作用,我们采用间接免疫荧光技术、ELISA夹心法及细胞培养,分别对15例患儿及对照组进行了T细胞亚群、Tac阳性细胞(mIL—2Rα)百分率、SIL—2R及IL—4含量检测。发现SIgAD患儿Tac阳性细胞(mIL—2Rα百分率及血清SIL—2R明显高于对照组,CD4、CD4/CD8、IL—4水平则显著低于对照组。表明血资SIL—2Rα异常增高不但可抑制IL—2的生物活性,还可反馈性地使其它免疫调节相关因子,如IL—4生成受阻。
In order to clarify possible roles of interleukin-2 receptor-(IL-2R)and interleukin-4 in pathogenesis of selective IgA deficiency (SIgAD), we detected the percentage of CD3,CD4, CD8and activated T Lymphocytes expressing IL-2R on the membrane with immunofluorescence, Serum IL-2with a sandwich ELISA and IL-4 in the culture media of peripheral mononuclear cells with an ELISA in 15 children with SIgAD and 15 normal controls. The results showed that the percentage of activated T cell positive for membrane IL-2R and serum IL-2R level were significantly higher than those of control group;while the CD4,CD4/CD8 and serum IL-4 level lever was significantly lower in SIgAD group than in the control group. These results suggest that the marked increase of serum IL-2 may not only inhibit the bioactivity of IL-2,but also down regulate generation of relevant cytokines such as IL-4 through a feedback effect.
