白细胞介素-6基因多态性对冠心病发病易感性的影响及其机制

Interleukin-6-597G/A and-572C/G polymorphisms and risk of coronary heart disease

目的了解白细胞介素-6（IL-6）基因-597G／A及-572C／G多态性对冠心病（CHD）发病易感性的影响及其影响机制。方法 应用聚合酶链反应．限制性片段长度多态性（PCR-RFLP）分析方法，测定245例CHD患者和260例正常对照者的IL-6基因型，探讨其与CHD的相关关系；观察基因型对血清IL-6水平的影响，并采用logistic回归分析法了解基因型与CHD其他危险因素间的相互作用。结果 两组研究对象中-597位点均仅发现GG基因型。-572C／G基因型和等位基因频率在两组间存在明显统计学差异（P均〈0．01），CHD组GG基因型和G等位基因频率均显著高于对照组（P均〈0．01）；CG、GG基因型人群患CHD的风险分别为CC基因型人群的1．46倍（95％CI：1．01～2．10，P〈0．05）和5．19倍（95％CI：1．69～15．89，P〈0．01）；不同基因型患者间血清IL-6水平无统计学差异（P〉0．05）；-572C／G基因型与总胆固醇、甘油三酯间存在一定的交互作用，OR值分别为1．76（95％CI：1．05～3．16，P〈0．05）、2．51（95％CI：1．04～6．45，P〈0．05）。结论 IL-6基因-597G／A多态性可能与中国汉族人群CHD发病易感性无关，而-572C／G多态性可能是该人群CHD发病的易感基因之一，其可能通过对组织IL-6水平的影响及与血脂的协同作用参与CHD的发生。

Objective To explore the relationship between interleukin-6 （IL-6） gene polymorphisms and the risk of coronary heart disease （CHD）. Methods IL-6/- 597 G/A and - 572C/G polymorphisms were genotyped in 245 CHD patients and 260 healthy adults by PCR-RFLP. Serum IL-6 level was examined by ELISA. Logistic regression was performed to observe the relationship between IL-6/- 572C/G polymorphism and other risk factors of CHD. Results IL-6/- 597G/A genotype was similar between the two groups. The frequencies of IL-6/-572C/G genotype and G allele were more frequent in patients with CHD than that in controls （P 〈0. 01 ）. Compared with CC genotype, the relative risk for CHD in people with CG and GG genotypes was 1.46 （95% CI： 1.01-2. 10, P 〈0. 05） and 5.19 （95% CI： 1.69-15.89, P 〈0. 01 ）, respectively. The serum levels of IL-6 were similar between carriers of the IL-6/- 572G allele and patients with CC genotype （ P 〉 0. 05 ）. IL-6/- 572 C/G is related to total cholesterol （ OR 1.76, 95% CI：1.05-3.16, P 〈 0.05） and triglyceride （OR = 2.51, 95% CI： 1.04-6.45, P 〈 0.05）, respectively. Conclusion IL-6/- 597G/A polymorphism was not associated with susceptibility to CHD, but IL-6- 572C/G polymorphism may be a possible genetic susceptibility factor for CHD in Chinese Hans population.