束缚应激影响大鼠四氧嘧啶性糖尿病发病的初步研究

An Inhibitory Effect of Restraint Stress on the Development of Diabetes Induced by Alloxan

将Ｗｉｓｔａｒ雄性大鼠束缚应激，腹腔注射６％四氧嘧啶（１００ｍｇ／ｋｇ），２４ｈ后血糖值为７．３±０．２ｍｏｌ／Ｌ，而未做应激处理的大鼠血糖值为９．９±０．６ｍｍｏｌ／Ｌ；既不应激也不注射四氧嘧啶动物的血糖值为７．０±０．６ｍｍｏｌ／Ｌ，说明束缚应激具有对抗四氧嘧啶致糖尿病的作用。以束缚应激后大鼠腹腔淋巴结提取的抑制因子给大鼠静脉注射，然后再腹腔注射同样剂量的四氧嘧啶。结果显示，注射淋巴结提取物大鼠血糖值为７．３±０．６ｍｍｏｌ／Ｌ，而单纯注射四氧嘧啶大鼠血糖值为９．８±０．８ｍｍｏｌ／Ｌ（Ｐ＜０．０５），说明束缚应激对抗四氧嘧啶致糖尿病作用可能由束缚应激产生的抑制因子所致。

Wistar rats were subjected to restraint stress overnight(15～18h),and then were intraperitoneally injected with 6%alloxan(100mg/kg).Blood glucose in control group(without stress)was 9.9±0.6mmol/L,however,blood glucose in experimental group was7. 3±0.2mmol/L. There is a statistically significant difference between them(P<0.01,t test).In order to study the role of the suppressive protein induced by restraint stress in the elevation of blood glucose induced by alloxan,a partially purified product of the protein derived from lymph nodes of stressed rats was intraperitoneally injected in rats.It was found that the elevation of blood glucose was suppressed. The results suggest that the suppressive protein induced by restraint stress may have an inhibitory effect on diabetes induced by alloxan.