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肝脏高效表达调控元件的构建及其表达效果的鉴定 被引量：2

Construction of a liver highly-effective control element and evaluation of its expression efficacy
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摘要 目的:构建一种肝脏高效表达调控元件,促进水动力转染外源基因在体内的长期高效表达。方法:构建了以hAAT基因作为报告基因,分别含有表达调控元件AerApoEAATP、ApoEAATP、A lbEAATP及CMV启动子的真核表达载体pC Ineo-AerApoEAATP-hAAT、pC Ineo-ApoEAATP-hAAT、pC Ineo-A lbEAATP-hAAT及pC Ineo-hAAT,水动力转染法将上述质粒分别转染BALB/c小鼠,定期检测小鼠血清中hAAT的浓度反应AerApoEAATP、ApoEAATP、A l-bEAATP及CMV的调控能力。结果与结论:所构建的表达调控元件AerApoEAATP具有较强的调控能力,其长期的调控水平高于CMV、A lbEAATP和ApoEAATP。 Objective： To construct a liver highly-effective control element that can prolong the expression time of hydrodynamically transfected gene. Methods： Plasmid pCIneo-AerApoEAATP-hAAT, pCIneo-ApoEAATP-hAAT, pCIneo-Al- bEAATP-hAAT and pCIneo hAAT were constructed respectively. All of them included hAAT gene as the reporter gene. BALB/c mice were transfected with the plasmids by hydrodynamical transfection technology. The activity of AerApoEAATP, ApoEAATP,AlbEAATP and CMV was compared by the concentration of hAAT. Results and Conclusion： The control element constructed is much stronger than ApoEAATP,AlbEAATP and CMV in the long-term expression.
作者 付秋霞 贾帅争 孙萍 杜娟 周勇 詹林盛 王会中 王全立
机构地区 北京输血医学研究所 解放军
出处 《军事医学科学院院刊》 CSCD 北大核心 2006年第3期206-208,229,共4页 Bulletin of the Academy of Military Medical Sciences
基金 国家自然科学基金 北京市自然科学基金资助项目(30300184 30400561 7063086)
关键词 BALB/C小鼠 Α1-抗胰蛋白酶 白蛋白 基因表达调控 肝 BALB/c mice α1 - antitrypsin albumin gene expression regulation liver
分类号 Q786 [生物学—分子生物学]
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参考文献6

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二级参考文献18

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共引文献5

同被引文献20

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军事医学科学院院刊
2006年 第3期

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