地黄多糖对小鼠免疫功能的影响

Effects of Rehmannia glutinosa polysaccharide on immune function of mice

目的:研究地黄多糖(RGPS)对正常小鼠免疫功能的影响。方法:以200、400、800 mg/(kg.d)剂量灌胃给药10 d,观察RGPS对小鼠胸腺指数、脾指数、吞噬指数、外周血CD4+和CD8+细胞的影响,采用体外给药的方法观察其对脾脏T淋巴细胞增殖、腹腔巨噬细胞吞噬活性的影响。结果:在一定的剂量范围内,RGPS可显著提高小鼠的脾指数,增强单核巨噬细胞的吞噬功能,提高CD8+细胞的比例,但对CD4+细胞无明显影响。体外实验表明,一定浓度的RGPS可明显促进T淋巴细胞增殖,增强腹腔巨噬细胞的吞噬活性。结论:地黄多糖可增强小鼠的非特异性免疫功能和细胞免疫功能。

Objective：To investigate the effects of Rehmannia glutinosa polysaccharide （RGPS） on immune function of mice. Methods： RGPS was ig. administered for 10 days at three doses of 200, 400, 800 mg/（kg · d） to observe its effects on the thymus index, spleen index, phagocytosis index, and peripheral CD4^ ＋ and CD8 ^＋ cells. At the same time, in vitro tests were used to evaluate its effects on the lymphocyte proliferation and phagocytic function of peritoneal macrophages. Results： With in certain dosage range, RGPS significantly increased the spleen index, strengthened the phagocytic function of mono-macrophage and elevated the percentage of CD8^ ＋ cells, but did not obviously influence the CD4^ ＋ cells. The in vitro tests indicated that RGPS at certain concentrations distinctly.improved the lymphocyte proliferation and phagocytic function of peritoneal macrophages. Conclusion： RGPS enhances the non-specific immunity and cellular immunity of mice.