精原干细胞移植及体外培养研究进展

Spermatogonial stem cells transplantation and culture:a research progress

精原干细胞(sperm atogon ial stem cells,SSCs)移植是近年来发展起来的一项技术,是将供体动物(正常动物或转基因动物)的SSCs移植到受体动物的睾丸内,使其在受体睾丸内迁移、定居、增殖并启动精子发生,产生有受精能力精子的过程。该技术在雄性不育的治疗、精子发生机制的探讨、干细胞生物学研究以及优质动物或转基因动物繁育等方面具有广阔的应用前景。SSCs的体外培养是制约该技术发展的瓶颈。近年来,许多研究者报道了SSCs体外培养的影响因子及其生长特点。本文就近年来SSCs的体外培养及移植的研究进展作了概要评述。

Spermatogonial stem cells （SSCs） transplantation is a novel technique developed in recent years, in which the testicular cell suspension from donor animals （ normal or transgenic animals） is microinjected into seminiferous tubes at the surface of the host testis, and subsequently, the donor SSCs survive, migrate, proliferate, initiate the spermatogenesis and produce the sperms with normal fertilization ability in the host testis. This technique provides a new opportunity for therapy of sterile male, study of spermatogenesis mechanism and production of transgenic animals. However, these applications are restricted by the in vitro culture of SSCs. In recent years, research into effects of cytokines on proliferation of SSCs were reported. Here the advances in SSCs culture and transplantation are reviewed.