芪桂益脉灵对豚鼠心室肌细胞钾离子通道活性的抑制作用

Inhibition of Qiguiyimailing on K^+ Channels Activity in Ventricular Myocytes

目的观察芪桂益脉灵对豚鼠心室肌细胞钾离子单通道的影响。方法实验应用膜片钳技术,采用细胞贴附记录模式,当灌注不同浓度的芪桂益脉灵原液时,观察钾离子通道的开放活性。结果芪桂益脉灵原液对钾离子通道的平均开放概率NP0值为0.11±0.04,对照组为0.62±0.17。芪桂益脉灵原液对钾离子通道的阻断作用在正常灌注液冲洗后可以恢复,冲洗后钾离子通道开放概率NP0值为0.41±0.12,与对照组相比无统计学意义(P>0.05)。结论芪桂益脉灵的抗心律失常作用与其有效抑制钾离子通道活性有关。

Objective To observe the effect of Qiguiyimailing on K^＋ channels activity in ventricle myocyte of guinea pig. Methods The patch - clamp recording technique was used to record the change of K ＋ channels activity at different dosage of Qiguiyimailing. Results Qiguiyimailing inhibited K^＋ channels significantly Compared with control group （ P 〈 0.05 ）. The K^＋ channels blockade by Qiguiyimailing could be recovered by perfusion solution washing. Conclusion The ant arrhythraic action of Qiguiyimailing appeared to relate its potent blocking K^＋ channels activity.