化妆品中10种邻苯二甲酸酯类化合物的高效液相色谱测定法

Determination of 10 Kinds of Phthalates in Cosmetics by High Performance Liquid Chromatography

目的建立化妆品中的10种邻苯二甲酸酯类化合物同时测定的高效液相色谱法。方法样品经甲醇溶解、超声提取、离心、过滤,采用C18(5μm,4.6mm×250mm)色谱柱,以甲醇-水为流动相梯度洗脱,流速为1.0ml/min,检测波长为280nm,柱温为25℃,高效液相色谱法进行测定,并探讨最佳实验条件。结果该方法各组分检出限如下,邻苯二甲酸二甲酯为0.1mg/L,邻苯二甲酸二乙酯为0.1mg/L,邻苯二甲酸二丙酯为0.5mg/L,邻苯二甲酸丁基苄基酯为0.5mg/L,邻苯二甲酸二正丁酯为0.5mg/L,邻苯二甲酸二戊酯为7mg/L,邻苯二甲酸二环己酯为7mg/L,邻苯二甲酸二己酯为7mg/L,邻苯二甲酸二乙基己基酯为1mg/L,邻苯二甲酸二正辛酯为1mg/L。该方法测定10种邻苯二甲酸酯的线性范围均为10～1000mg/L,相对标准偏差小于3.95%(n=6),加标回收率在98.59%～108.07%之间。结论本法操作简便、准确、快速,可同时测定化妆品中的10种邻苯二甲酸酯类化合物。

Objective To establish a high performance liquid chromatography method for simultaneous determination of 10 kinds of phthalates in cosmetics. Methods 10 kinds of phthalates were separated on a C18 column using methanol-water as mobile phase at a flow rate of 1.0 ml/min, 25℃ column temperature and 280 nm detection wave. Results The detection limit of 10 kinds of phthalates were 0.1-7 mg/L. The precision was less than 3.95% and recovery rates varied from 98.59% to 108.07%. Conclusion The experimental results show that the method is simple, precise and accurate and suitable to simultaneous determination of 10 kinds of phthalates in cosmetics.