我国部分地区近年来新城疫病毒流行株分子生物学特征的研究

Molecular Biological Characterization of Newcastle Disease Viruses Isolated from Different Geographical Locations in China

参考GenBank中的新城疫病毒（NDV）的基因序列，在F基因的3’端相对保守区设计1对引物，利用RT-PCR方法对华南农业大学兽医学院禽病学教研室近几年来分离并保存的24株新城疫病毒毒株的F基因进行了扩增，其扩增长度为450bp．测序结果表明：近十几年来，我国流行的新城疫毒株主要为基因Ⅶ型，其氨基酸序列均符合F蛋白裂解位点区（112～117）强毒株的氨基酸序列特征，并从分子水平上进一步证明我国最近流行的NDV主要为基因Ⅶ型NDV强毒株．

Twenty four field strains of newcastle disease viruses （NDV） isolated from different geographical locations in China in the last 10 years were characterized genetically. A pair of primers specific to NDV gene F 3＇ end was designed according to the known sequences from GenBank, and was used to amplify the NDV cDNA gene F 3＇ end by RT-PCR from total RNA, which was 450 bp in length. Analysis of the F gene sequence revealed that 20 strains belonged to the genetype Ⅶ, whose amino acid sequences of the cleavage site region （112-117） were similar to that of all other virulent strains. The results confinned that the majority of epidemic strains of NDV in China recently are virulent.