宫内感染和窒息联合作用对胎鼠脑损伤及细胞凋亡和胶质纤维酸性蛋白表达的影响

Effect of Intrauterine Infection and Interuterine Asphyxia to Fetal Rats′ Brain Damage,Cell Apoptosis and Expression of Glial Fibrillary Acidic Protein

目的了解宫内感染和窒息联合作用对胎鼠脑损伤、细胞凋亡程度和胶质纤维酸性蛋白(GFAP)表达影响。方法妊娠18 d SD孕鼠随机分为4组:1.NS+假手术组;2.宫内感染组;3.宫内窒息组;4.宫内感染+宫内窒息组。于不同处理后72 h取胎鼠脑组织,分别进行HE染色、TUNEL和GFAP免疫组织化学镜检。结果宫内感染+窒息组较单纯宫内感染组或宫内窒息组细胞水肿程度、组织紊乱程度重。TUNEL和GFAP染色结果提示阳性细胞数在宫内感染+窒息组>宫内感染组、宫内窒息组>NS+假手术组,前3组与NS+假术组比较均有统计学差异(P=0),宫内感染+窒息组与单纯宫内感染组、宫内窒息组比较均有统计学差异(P=0)。结论宫内感染和窒息均可导致胎鼠脑损伤,宫内感染和窒息同时存在时能加重两者分别单独作用时的脑损伤程度,使凋亡和GFAP阳性细胞数目增加。

Objective To find out the associated effect of intrauterine infection and interuterine asphyxia to fetal rat＇s brain damage, cell apoptosis,and expression of glial fibrillary acidic protein（GFAP）. Methods Pregnant rats of gestation 18 days were randomly divided into four groups： 1 - NS plus sham operation, 2. intrauterine infection, 3. intrauterine asphyxia, 4. intrauterine infection plus intrauterine asphyxia. The fetal rats＇ brains were taken out 72 h after different disposal and given HE coloration, immunohistochemistry of TUNEL and GFAP, respectively. Results The level of brain cell edema and tissue disorganization of group intrauterine infection plus intrauterine asphyxia were more serious than those of group intrauterine infection or group intrauterine asphyxia. TUNEL and GFAP had the .same resuits：The number of positive cells in group intrauterine infection plus intrauterine asphyxia more than that in group intrauterine infection, and which in group intrauterine asphyxia more than that in group NS plus sham operation. There was significant difference between the first three groups and the group .NIS plus sham operation（ P = 0）. There was also significant difference between group intrauterine infection plus intrauterine asphyxia and group intrauterine infection or group intrauterine asphyxia （ P = 0）. Conclusions Both intrauterine infection and intrauterine asphyxia may induce premature rat brain damage, the association of intrauterine infection and intrauterine asphyxia may aggravate the degree of fetal rat brain damage, also increase the number of apoptosis cell and the expression of GFAP.