湖南省2001～2005年急性弛缓性麻痹病例病毒学监测结果分析

Analysis of the Result of Rirological Surveillance on AFP Cases in Hunan Province in 2001-2005

目的评价湖南省2001～2005年急性弛缓性麻痹（AFP）病例病毒学监测情况，巩固无脊髓灰质炎（脊灰）成果。方法收集〈15岁AFP病例粪便标本，采用世界卫生组织规定方法进行肠道病毒（EV）分离与鉴定，脊灰病毒阳性株送中国疾病预防控制中心病毒病预防控制所国家脊灰实验室进行型内鉴别。结果5年共检测1103例AFP病例粪便标本（2185份），EV分离阳性276例，未发现脊灰野病毒，分离到疫苗相关脊灰病毒112例，疫苗变异脊灰病毒6例，疫苗相关脊灰病毒与非脊灰肠道病毒（NPEV）混合4例，NPEV154例。结论湖南省2001～2005年未发现脊灰野病毒，维持了无脊灰状态。但监测发现疫苗变异脊灰病毒6例，必须采取有效措施阻止可能的疫苗衍生脊灰病毒的循环和爆发。

Objective Using AFP case surveillance system and virological surveillance, Hunan Province reached the target of polio-free during 2001 - 2005. Methods Stool samples of those AFP cases less than 15 years old were,, collected. Virological isolation and identification were carried out in accordance with WHO standards, and all polio-positive strains were sent to National Poliomylitis Laboratory for type determination. Results In five years, a number of 2 185 stool specimens of 1 103 AFP cases had been examined, 276 were identified as enterovirus positive. Among the positive specimens, 112 were identified as vaccine-related polioviruses,6 were identified as vaccine-variant polioviruses,4 were mixed type of vaccine-related polioviruses and non-polio enterovirus, 154 were non-polio enteroviruses. Conclusion The results of surveillance indicates that all the indicators of AFP surveillance of Hunan Province have reached and kept the above target of WHO＇ s requirements. No wild poliovirus confirmed that Hunan Province sustain polio-free status. However, the high variant poliovirus of 6 vaccinevariant polioviruses warned us that we should take effective measures to prevent circulation and outbreak of vaccine-derive.d polioviruses （VDPV）.