吉林省乙型肝炎病毒基因型分型及临床研究

Genotyping of Hepatitis B Virus in Jilin Province and its Clinical Investigation

目的了解吉林省乙型肝炎病毒(HBV)的基因型流行及分布情况,探讨HBV基因型与其预防治疗的临床意义。方法采用多对型特异性引物套式聚合酶链反应(nest-PCR)对吉林省194份HBV阳性血清进行分型,并研究HBV与预防和治疗的关系。结果在194例HBV血清阳性标本中,B型16例,C型172例,B、C混合型6例,未发现其它型别的病毒。结论在吉林省HBV的基因型主要为C型,B、C混合型也占了一定比例。C型与肝硬化和肝癌关系密切,混合型与慢性肝炎关系密切。

Objective To understand the distribution of hepatitis B genotype in Jilin Province and its clinical significance. Methods Based on nest-PCR, using type-specific primers for the determination of genotype from 194 share of positive blood serum in Jilin Province. Results The genotype B was 16 samples,C was 172 samples, BC was 6 samples and did not fund others genotypes. Conclusions The major genotype in Jilin Province was genotype C;and the genotype BC was a definite proportion.