金纳多对脑缺血再灌注大鼠神经细胞凋亡和caspase-3表达的影响

Effects of Ginaton on Neural Cells Apoptosis and Expression of Caspase-3 in Rat Cerebral Ischemia-reperfusion

目的探讨金纳多对大鼠脑缺血再灌注后神经细胞凋亡及caspase-3表达的影响。方法线拴法制作大鼠脑缺血再灌注模型;30只健康成年SD大鼠随机分3组:假手术组、模型组、金纳多治疗组;每组于脑缺血2h再灌注24h行脑灌注固定取材,采用苏木素-伊红(HE)染色以观察梗死灶的部位和范围;采用原位末端标记法(TUNEL)及免疫组化染色检测脑组织凋亡细胞数及caspase-3蛋白表达。结果与其它两组相比,金纳多治疗组脑组织病理变化明显减轻,神经细胞凋亡、caspase-3蛋白表达明显减少,差异有统计学意义(P<0.05)。结论金纳多对大鼠缺血性脑损伤有保护作用,下调caspase-3蛋白表达减少神经细胞凋亡可能是其主要机制之一。

Objective To investigate the effects of Ginaton on the apoptosis of neural cells and the expression of caspase -3 in rat cerebral ischemia -reperfusion. Methods Rats cerebral ischemia- reperfusion model was established with suture emboli method; 30 healthy adult male SD rats were divided into sham operated group, model group, Ginaton treated group at random. Rats＇cerebral tissue was obtained in 24h after stroke. Part and area of infarct were investigated by HE dyeing. The brain sections were used for terminal deoxyuncleotidyl transferase -mediated dUTP -biotin nick -end labeling（TUNEL） staining and caspase -3 immunohistochemical staining. Results The apoptosis of neural, the expression of caspase - 3 in Ginaton treated group significantly decreased as compared with that of the control group （ P 〈 0. 05 ）. Significant recorvery of pathologic change was found in rats treated with Ginaton compared with control rats. Conclusion The therapy of Ginaton on rat brain following cerebral ischemia is effective. It was one of the main protection mechanisms of Ginaton by down - regulating caspase - 3 that could reduce apoptosis of nerve cells.