RT-PCR技术在肠道病毒脑膜炎暴发中的应用

Application of RT-PCR Technique on Outbreak of Enterovirus Encephalitis

目的应用套式RT-PCR确定疫情暴发的病原,为制定预防控制措施提供实验室支持。方法提取患者脑脊液和血清中的病毒RNA,用肠道病毒特异性引物进行套式RT-PCR扩增,检测特异性基因片段。结果14份血清和6份脑脊液标本中分别有2份标本肠道病毒特异性基因片段扩增呈阳性。结论套式RT-PCR检测患者脑脊液或血液中的肠道病毒特异性基因片段具有快速、特异的特点,是一种适合突发疫情处置的快速诊断技术。

Objective To determine the pathogen causing outbreak with RT -PCR in order to implement preventive measures. Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT - PCR so as to detect specific gene fragment. Results Respectively 2 samples were positive on specific gene fragment of 14 samples of sera and 6 samples of cerebrospinal fluid. Conclusion RT - PCR is a kind of technique to determine pathogen causing outbreaks since it can be used to detect specific gene fragment in cerebrospinal fluid or blood of patients rapidly.