益气化瘀方及拆方对椎间盘软骨细胞Ⅰ、Ⅱ型胶原的影响

Effects of "Yiqi Huayu Recipe" and Its Decomposed Formulas on Type-Ⅰand Type-Ⅱ Collagens of Enplate Chondrocytes on Rats

目的探讨益气化瘀方及拆方对椎间盘软骨细胞凋亡状态下Ⅰ、Ⅱ型胶原表达的调控作用。方法用酶消化法培养大鼠椎间盘软骨细胞,抗Fas抗体诱导凋亡,通过益气化瘀方及拆方益气方、化瘀方药理血清干预,免疫组化法(SP)检测分析大鼠椎盘软骨细胞不同状态下Ⅰ、Ⅱ型胶原的表达情况。结果相对正常对照组,凋亡模型组的I型胶原表达明显,而II型胶原轻度表达,有显著差异(P<0.01)。相对凋亡模型组,益气化瘀方、益气方和化瘀方均能上调II型胶原的表达,下调I型胶原表达,3种中药组与诱导凋亡组相比有显著差异(P<0.01)。结论益气化瘀方及拆方可以调控凋亡大鼠椎间盘软骨细胞内I、II型胶原表达。

Objective To study the effects of ＂Yiqi Huayu Recipe＂ and its decomposed formulas-medicated sera on expressions of type-Ⅰ and type-Ⅱ collagens of apoptotic endplate condrocytes in rats. Methods Immunohistochemical and integral optical density analytic methods were used to observe the effects of ＂Yiqi Huayu Recipe-＂, Huayu Recipe-medicated sera on the expressions of type-Ⅰ and type-Ⅱ collagens of apoptotic endplate condrocytes in rats induced by anti-Fas antibody. Results As compared with apoptosis group,type-Ⅱ collagen expression was higher, type-Ⅰ collagen expressions were lower in Yiqi Huayu-treated Yiqi-treated, Huayu-treated （P〈 0.01 ）. Conclusion ＂Yiqi Huayu Recipe＂ and its decomposed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action on regulating the expressions of type- Ⅰ and type- Ⅱ collagens.