黄芪减轻砒石对大鼠肝、肾毒性作用研究

THE EFFECT OF ASTRAGALUS MEMBRANCCUS ANTAGONISTIC ACTION ON THE TOXICITY OF ARSENOLITE TO LIVER AND RENAL FUNCTION

目的为研究中药黄芪对砒石致毒大鼠体内肝、肾功能的影响,进一步探讨黄芪减轻砒石对大鼠的毒性作用。方法50只SD大鼠随机分成5组,即(A组)空白对照组(正常对照组);(B组)砒石对照组(10mg/kg);(C组)砒石加黄芪(3.8g/kg)低剂量组;(D组)砒石加黄芪(7.6g/kg)中剂量组;(E组)砒石加黄芪(15.2g/kg)高剂量组。每天灌胃给药1次,连续喂养12w后处死。通过检测增加黄芪治疗后的大鼠血清丙氨酸氨基转移酶(ALT)、血尿素氮(BUN)、血清肌酐(SCR)含量的变化。结果砒石作用大鼠机体后,血清ALT、BUN、SCR升高,黄芪与砒石同服后,能显著降低ALT、BUN、SCR等值,与砒石对照组比较差异有统计学意义(p<0.05)。结论黄芪对减轻砒石致大鼠肝、肾组织损害有一定的作用,对砒石的毒性作用可能起到一定程度的解毒作用。

Objective To investigate the possible mechanisms of oral administration of astragalus membranceus, which decreases arsenolite - induced toxic action in liver and renal function. Methods 50 healthy SD rats weighted （120 ±20）g were randomly divided into 5 groups （n = 10） ： group A （normal sodium group, normal control group）, group B （arsenolite group, control group）, group C （arsenolite plus low dose of astragalus membranccus group ）, group D （ arsenolite plus middle dose of astragalus membranccus group）, group E （ arsenolite plus high dose of astragalus membranccus group）, Created the toxic model respectively, the rats were killed and sampled after 12 weeks, alanine aminotrans- ferase（ ALT）, blood urea nitrogen （BUN） and serum ereatinine（SCR） in serum were detected by eolorimetrie method. Results Oral administration of astragalus membraneeus after arsenolite, the level of ALT, BUN, SCR are decreases remarkably, the necrosis and cellular edema of liver cell decrease obviously on pathology, the amount of index above in groups D were significantly lower than those in group B （p 〈 0. 01 -p 〈 0. 001 ）. Conclusion Oral administration of astragalus membranccus decreases obviously the damage of liver and renal function which arsenolite induced in rats.