摘要
目的 建立了用HPLC测定倍他米松搽剂中倍他米松的含量.方法 色谱柱为C18柱(4.6mm×250mm,5μm),流动相为甲醇-水(70:30),流速为1.0ml·min^-1,检测波长为240nm.结果 倍他米松的线性范围为40~120μg·mL^-1(r=1.000),平均回收率为100.0%,RSD为0.5%.结论 该方法简便、准确、快速、重复性好.
OBJECTIVE To establish an HPLC method for the determination of Betamethasone in Betame thasone Liniment. METHODS The analytical column was C18column(4.6mm×250mm,5μm),and the mobile phase was methanol-water (70:30)at a flow rate of 1.0ml· min^-1 .Detected at 240nm. by UV- detector. RESULTS The linearity was good for Betamethasone within the range of 40~120μg·mL^-1 (r= 1. 000) , the mean recovery was 100. 0%(n = 9), RSD= 0. 5 %. CONCLUSION The results show that the method was simple precise and rapid.
出处
《海峡药学》
2006年第3期54-55,共2页
Strait Pharmaceutical Journal