高效液相色谱法测定石榴皮中没食子酸的含量

Content Determination of Gallic Acid in Megranates pericarp by HPLC

目的 测定石榴皮中没食子酸的含量.方法 采用高效液相色谱法,Zorbax-C18色谱柱,流动相：水-二甲基甲酰胺-冰醋酸（99.82：0.15：0.03）,检测波长270nm,流速1mL·min^-1,柱温为室温.结果 在0.081～0.811μg·mL^-1范围内,没食子酸峰面积（Y）与其进样量（X）呈良好的线性关系（r=0.9999）,平均日内、日间RSD为1.01%和1.60（n=6）,不同浓度的平均回收率为98.35%,RSD=1.63%（n=6）.结论 该法快速简便,稳定可靠,专属性强.

OBJECTIVE To determine the content of gallic acid in Megranate＇s-pericarp with HPLC. METHODS A zorbax-C18 column （250mm ×4.6mm, 5μm） was used. The mobile phase was waterdimethyformamide-glacial acetic acid（99.82：0. 15：0. 03）,and detected at 270nm with flow rate of 1.0mL·min^-1 ,and the temperature of column was at room temperature. RESULTS The linearity of gallic acid was over the range of 0. 081～0. 811μg· mL^-1 with the correlation coefficient 0. 9999. The average recoveries of different concentration was 98.35% with RSD 1.63% （n=6）;The within-clay and between-day RSD was 1.01% and 1. 60% （n=6）. CONCLUSION The method is convenient stable reliable and special for the dermination of gallic acid in Megranate s-pericarp.