摘要
目的 建立测定人血浆中奥氮平浓度的反相高效液相色谱法.方法 以C18反相柱（150mm×4.6mm, 5μm）为色谱柱,流动相0.03mol·L^-1醋酸铵-甲醇（35：65）;流速：0.8 ml·min^-1;柱温：40°C;检测波长： 275nm.以乙酸乙酯与二氯甲烷（80：20）为提取剂.结果 奥氮平320.0, 80.0,10.0μg·L^-1 3种浓度平均回收率分别为102.92%, 97.11%,98.20%,日内、日间差RSD均低于6%（n=5）;最低检测浓度为5.0μg·L^-1;线性范围为5.0～320.0μg·L^-1.回归方程为：C=264.03F-1.21,r=0.9996（n=9）.结论 本法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究.
OBJECTIVE To establish a method for determining the plasma concentration of olanzapine by HPLC . METHODS Olanzapine was extracted with diethyl ether. The residues were analyzed with a reverse phase HPLC system Diamonsil C18 column （150mm ×4.6mm, 5μm） with the mobile phase consisted of 0.03mol·L^-1NH4AC-Methanol （35：65）. The detection wavelength was at 275nm. RESULTS The average recoveries of olanzapine were 102.92%, 97.11% and 98.20%, respectively. The within-day and between-day precision of variation was less than 6 %. The calibration curves of olanzapine had good linearity, r= 0. 9996 （n= 9）, over the range of 5.0～320. 0μg·L^-1 CONCLUSION The method provides a sensitive, accurate precise and reliable analytical procedure for clinical monitoring of olanzapine in plasma and its pharmacokinetics studies.
出处
《海峡药学》
2006年第3期184-186,共3页
Strait Pharmaceutical Journal
