远程氩等离子体改性膜固定化脲酶的研究

Study on immobilization of urease on PTFE membrane modified with argon remote-plasma

利用远程Ar等离子体技术引发聚四氟乙烯(PTFE)膜表面接枝丙烯酸,并以此膜为脲酶的固定化载体。研究结果表明:在放电压力20 Pa、放电功率100 W、放电时间100 s的等离子体处理条件和接枝温度70℃、接枝时间50 h、单体体积分数26%的接枝条件下,PTFE膜表面丙烯酸接枝量为131.7μg/cm2;固定化脲酶接枝密度随PTFE膜接枝丙烯酸(AA)量的增加几乎呈线性上升,固定化酶活性开始随PTFE膜接枝AA量的增加而增加,随后趋于平缓增加。同时评价了固定化酶膜的稳定性,并对接枝膜和固定化酶膜的结构进行了表征。

Polytetrafluoroethylene（PTFE） film was treated by argon remote-plasma and grafted with acrylic acid（AA）. Then urease was immobilized onto this film as immobilization carrier. It was shown that AA grafting yield onto PTFE film surface was 131.7 μg/cm^2 ,after 100 W and 100 s of plasma treatment under 20 Pa,and 50 h of graft polymerization at 70℃ with 26% of AA volume fraction. The graft density of immobilized urease was a linear relationship which increased with AA graft yield on PTFE film. The activity of immobilized urease increased with AA graft yield on PTFE films and then turned to level off. The stability of immobilized urease membrane was evaluated and the structure and composition of the grafted film and immobilized urease membrane characterized.