热痛方对大鼠急性肺损伤保护作用的实验研究

THE PROTECTIVE EFFECTS OF RETONG FANG ON ACUTE LUNG INJURY IN RATS

目的了解热痛方对大鼠急性肺损伤的保护作用机制。方法40只健康成年SD雄性大鼠随机分为4组:生理盐水组(NS组)、模型组(ALI组)、热痛方组(RT组)和地塞米松组(DM组)。除NS组外,每组大鼠尾静脉注射脂多糖(LPS,5mg/kg)复制急性肺损伤模型,并于注射LPS2 h后行腹腔麻醉,腹主动脉采血处死。测肺系数、外周血白细胞与血小板数,行肺泡灌洗后测灌洗液中的白细胞数及TNF-α的含量。光镜下观察肺组织形态学变化。结果应用热痛方预先给药可明显减轻内毒素引起的肺组织形态学改变。与ALI组相比,RT组肺系数、肺泡灌洗液中白细胞数及TNF-α含量均显著降低(P<0.01或P<0.05),外周血白细胞数升高(P<0.05);与DM组相比,肺系数、外周血小板计数、肺泡灌洗液中白细胞数存在差异。结论热痛方预先给药可能通过减轻肺部的炎性渗出、改善肺部的局部微循环、抑制炎症因子TNF-a的产生等途径减轻内毒素引起的急性肺损伤。

Objective To investigate the protective effects of Retong Fang on acute lung injury （ALI） induced by Lipopolysaccharide （LPS） in rats. Methods Forty SD rats were randomly divided into 4 groups： sodium chloride group （n = 10,with sodium chloride 2.5ml/kg injection） ;LPS model group （n = 10,with LPS 5mg/kg injection） ; Retong Fang treated group （n = 10, gavage with 1 g/kg Retong Fang 24,12, and 1 hours before LPS injection）, and Dexamethasone treated group （n = 10, with 4mg/kg Dexamethasone injection 1 hour before LPS injection ）. All the animals were killed at the time point of 2h after LPS injection. Blood samples were obtained from abdominal artery for determination of WBC, BPC count, and the changes with lung coefficient （ LC ） , total cell counts in bronchial velour lavage fluid （BALF） , and lung pathology were observed, contents of tumor necrosis factor-α （ TNF-α ） were measured by ELISA. Results The levels of BPC counts, BALF TNF-α, LC, total cell counts in BALF, in Groups Retong fang were markedly lower than those in Group ALI（ P 〈0.01 or P 〈0.05） ,lower than those in Group Dex （ P 〈 0.05 ）. Conclusion Retong fang has therapeutic and protective effects on acute lung injury.