原因不明习惯性流产免疫治疗前后血清TGF-β_1变化研究

Study of immunotherapeutical influence on unexpiained habitual abortion patients serum transforming growth factor beta 1.

目的检测原因不明习惯性流产(UHA)患者免疫治疗前后血清中TGF-β1变化,探讨其在UHA发病及治疗中的作用。方法采用皮下注射丈夫淋巴细胞的方法治疗UHA患者;应用ELISA法检测正常非孕妇女30例和UHA妇女30例免疫治疗前后血清TGF-β1含量(Pg/mL)。结果UHA患者血清中TGF-β1含量(10178.50±3258.60)明显低于正常非孕妇女(11988.10±3423.65),P<0.025=;24例妊娠成功者治疗后血清中TGF-β1含量(12801.21±3510.97),与治疗前相比明显升高,(P<0.025=;6例妊娠失败者治疗后血清中TGF-β1含量(11079.50±3227.21),与治疗前比较无明显变化,(P>0.05)。结论血清中TGF-β1水平降低可能与UHA的发生有关,免疫治疗可上调血清中TGF-β1水平,诱导母体免疫耐受,使妊娠成功。

Objective： To detect the serum TGF-β1 in unexplained habitnal abortion （UHA） patients before and after the immunotherapy inorder to explore the function TGF-β1 in the genesis and treatment of UHA. Methods ： Applied ELISA method to detect serum TGF-β1 before and after hypodexmic injection of the couples＇ lymphocytes in 30 UHA patients and 30 healthy non-pregnancy women. Results： TGF-β1 in UHA patients （10178.50±3258. 60） has significantly reduced than that in healthy non-pregnancy women （11988. 10±3423. 65）, （ P 〈0. 025 = ; TGF-β1 in 24 successful pregnancy women （12801.21± 3510. 97） has significantly elevated after treatment, （ P 〈0. 025 = ; otherwise TGF-β1 in 6 unsuccessful pregnancy women after treatment （ 11079. 50±3227. 21 ） has no significant changes （ P 〉 0. 05）. Conclusion： Reduced serum TGF-β1 is probabty related to the genesis of UHA, Immunotherapy may elevate the level of serum TGF-β1 which call induce maternal immunotolerance and acquire successful pregnancies.