睾丸酮丛毛单胞菌降解胆固醇的培养条件研究

Conditions of Culture of the Comamonas Testosteroni Degrading Cholesterol

利用睾丸酮丛毛单胞菌(菌株c.test+act5、菌株c.test+tac、菌株c.test)具有消化甾醇这一特性,以胆固醇为底物筛选其降解甾醇的培养条件,使胆固醇降解酶的活力相对提高,从而为高效降解甾醇类物质的工程菌的筛选奠定基础.研究结果:最佳诱导时间14 h,培养基最佳pH7.0、最佳诱导温度30℃、及最佳装液量50 ml(250 ml摇瓶).在此条件下胆固醇降解酶活力:菌株c.test+act5为3.82×10-3U.ml-1、菌株c.test+tac为3.51×10-3U.ml-1、菌株c.test为2.68×10-3U.ml-1.

This research was degrading the cholesterol by Comamonas Testosteroni （strain ：c. test ＋ act5, c. test ＋ tac, c. test）that can digest sterol, screening the conditions of degrading the sterol. The results were summarized as following： screening the best inducement hour was 14 h, the best pH was 7.0, the best inducement temperature was 30℃, the best volume of culture medium was 50 ml of 250ml bottle. On the base of these conditions , the activity of c. test ＋ act 5 is 3.82× 10^-3U·ml^-1,c. test ＋ tac is 3.51 × 10^-3U·ml^-1, c. test is 2.68× 10^-3U·ml^-1.