PTCA球囊封堵冠状动脉制作猪急性心肌梗死再灌注模型及判定

Establishment and identification of swine model of acute myocardial infarction by coronary balloon occlusion with PTCA technique

目的:探讨PTCA球囊封堵猪冠状动脉建立急性心肌梗死再灌注动物模型的实验方法并进行判定。方法:选用中华小型猪8只,将PTCA球囊放至冠状动脉左回旋支第一钝缘支,堵闭血流90 m in,再灌注60 m in后,用坏死特异性对比剂(EC-60)增强MR I在活体确定梗死区;离体标本进行氯化三苯基四氮唑(TTC)组化染色验证梗死区。结果:成功建立8只猪急性心肌梗死动物模型,活体EC III-60增强MR I高信号区与离体TTC染色所示梗死区部位与面积一致(P>0.05)。结论:应用PTCA球囊封堵猪冠状动脉可成功建立急性心肌梗死动物模型,这种模型具有重复性好、可控性强的优点,且创伤较小,接近临床病理生理过程,可作为急性心肌梗死研究的技术平台。

Objective To investigate and identify a swine model of acute myocardial infarction （AMI） by coronary occlusion with angioplasty balloon. Methods After anesthesia, eight swines underwent 90 minutes of occlusion and 60 minutes of reperfusion of the obtuse marginal branch of the left circumflex（LCX） coronary artery by inflating and deflating a transcatheter balloon. The in vivo MR images enhanced with a necrosis-avid contrast agent （EC-60） and postmortem 2,3,5-triphenyltetrazolium chloride （TTC） histochemical staining were performed to confirm AMI. Results The AMI were successfully induced in all 8 swines. The distribution and mean EC-60 induced hyperintense areas on in vivo MR images were consistent with that of AMI as identified by using TIC staining （ P 〉 0. 05 ）. Conclusions A swine model of AMI by coronary occlusion with angioplasty balloon could be established effectively, which proves to be reproducible, controllable, andminimally invasive. This model mimics human conditions and should be feasible for pertinent experimental research.