硒和镁拮抗二氧化硅致肺泡巨噬细胞氧化损伤的研究

Effect of sodium selenite and magnesium sulfate in antagonism to the oxidative injury of silica in alveolar macrophages

目的:观察亚硒酸钠与硫酸镁单独及联合应用对染尘肺泡巨噬细胞丙二醛(MDA)、过氧化氢(H2O2)及过氧化氢酶(CAT)水平变化的影响,寻找二者的最佳剂量组合。方法:采用大鼠肺灌洗法纯化获得肺泡巨噬细胞(1×109L-1),同时加入S iO2及不同浓度的亚硒酸钠溶液、硫酸镁溶液、亚硒酸钠+硫酸镁溶液,于37℃、5%CO2培养条件下培养18 h后,检测MDA、H2O2含量及CAT活性。结果:亚硒酸钠与硫酸镁单独以及两者联合应用都可以使S iO2染毒的肺泡巨噬细胞MDA及H2O2含量下降,CAT活性有所增高,且以1.0μmol.L-1亚硒酸钠与1.0μmol.L-1硫酸镁联合作用效果最好。结论:亚硒酸钠与硫酸镁在体外可有效拮抗S iO2粉尘的细胞毒性作用,二者合用效果更佳。

Objective To investigate the effect of sodium selenite and magnesium sulfate used either solely or together in antagonism to the toxicity of silica on alveolar macrophages. Methods Alveolar macrophages were cultured with silica, at the same time sodium selenite, magnesium sulfate and zinc gluconate together with magnesium sulfate were added respectively. Malondialdehyde （ MDA）, hydrogen peroxide （ H2O2） and catalase （CAT） in alveolar macrophage were measured. Results sodium selenite, magnesium sulfate, sodium selenite together with magnesium sulfate had the effects of antagonizing the toxicity of silica on alveolar macrophages. The most suitable combination of the two substances should be 1.0 μmol · L^-1 of sodium selenite with 1.0 μmol · L^-1 of magnesium sulfate. Conclusions Sodium selenite and magnesium sulfate used both solely or together can reduce the levels of MDA and H2O2 and increase activity of CAT in alveolar macrophages exposed to silica.