针刺预处理抗脑缺血再灌注损伤作用及其分子机制

Research on the Influence and Molecular Mechanism of Acupuncture Preconditioning in Rats With Temporary Cerebral Isehemia Reperfusion

目的:研究针刺预处理对脑缺血再灌注神经元损伤的“治未病”作用及其神经生物学分子机制。方法:以肾俞、百会穴为针刺用穴;颈动脉引流法全脑缺血7m in再灌注造模;病理学石蜡包埋切片,HE染色,进行顶皮质I区V层幸存神经元记数,观察脑片缺血性病理变化;基因芯片技术进行针刺诱导的大鼠脑组织基因表达差异分析。结果:不同再灌时间段,E0.5h组幸存神经元密度显著均高于D组、E1.5h组和E3h组(P<0.05),E3h组与D组无差异(P>0.05)。针刺预处理后0.5h,大鼠脑组织表达变化大于2倍的基因共265个,20个基因表达差异具有显著意义,其中8个基因表达上调,12个基因表达下调。结论:针刺预处理具有抗脑缺血再灌注损伤作用,该作用具有明显的时间依赖性;一些针刺诱导基因表达产物在这一过程中发挥了重要作用;本研究结果为针灸“治未病”提供了形态学及分子生物学实验证据。

Objective： To observe the resist -injury effect and molecular mechanism of aeupuneture preconditioning（AP） ineerebral cortex of temporary cerebral isehemia reporfusion rats. Methods： 101 Wistar rats randomly divided into normal control group （ A group, n = 8）, sham - isehemia control group （ B group, n = 1 5 ）, AP control group （ C group, n = 18 ）, cerebral isehemia reperfusion control group（ D group, n = 15）, AP and cerebral isehemia reperfusion group（E group, n =45）. A rat model of temporary cerebral isehemia reperfnsion injury by blood drainage from arterial of earotis communis was used to compare the influences on the density of survive neuron in cerebral cortex after eleetreaeupuneture of aeupionts ＂Shenshu＂ （1323） and ＂Baihui＂ （ DM20）. The density of survive neuron were detected with HE stain method. High - density oligonueleotide mieroarrays.used to analyze gene expression in the brain. Results： The density of survive neuron in E0.5h group were signifieandy higher than D group and E1.5h group and E3h group at different time（ P 〈0.05） ; hut there were no significant differences between D group and E3h group at all time（ P 〉 0.05 ）. Expression of a large number of genes changes in response to AP, many of which can be linked to neuronal signaling and strueture, transcription regulation, receptor and transmission. Conclusion： The results of the present study can lead to a conclusion of AP can resist the injury induced by temporary cerebral isehemia reperfusion. This effect was better at 30 rain after AP than 90 min and 180min. A number of these genes may play important roles in the effect.