六味地黄汤对实验性骨关节炎兔软骨细胞凋亡与增殖的体内实验

Effect of Liuwei Dihuang Decoction on the Chondrocyte Apoptosis and Proliferation of Experiment Rabbit Osteoarthritis

目的:探讨六味地黄汤对实验性膝骨关节炎兔软骨细胞增殖、凋亡的影响。方法:20只新西兰兔用Hu lth法造骨关节炎动物模型,随机分成空白对照组、实验组;实验组用六味地黄汤灌胃,空白对照组用生理盐水,治疗第4、8、12、24周后每组各取2只动物,用亚硝酸盐还原酶法测定关节滑液NO水平,关节软骨组织HE染色,光镜观察形态学变化、电镜观察软骨细胞内结构变化及原位末端标记(TUNEL)法观察软骨细胞凋亡病理改变,进行组织病理学评估。结果:空白对照组NO活性在不同时期均高于实验组(8周,P<0.05;12、24周,P<0.01),两组间比较有显著性差异(P<0.05,0.01);实验组的关节软骨病理变化总积分、软骨细胞病理改变积分和软骨表层病理改变积分均明显低于空白对照组(P<0.05);实验组的软骨细胞凋亡指数与对照组的软骨细胞凋亡有显著性差异(P<0.05),与关节液中NO含量显著相关。结论:六味地黄汤对兔膝关节实验性骨关节炎软骨退变具有保护作用,通过NO诱导途径减少软骨细胞的凋亡,可能是其作用机制之一。

Objective： To investigate the effect of Liuwei Dihuang Decoction on the chondrocyte apoptesis and proliferation of experiment rabbit osteoarthritis. Methods： Twenty experimental osteoarthritis New Zealand adult rabbits which established by Hulth method, were randomly divided into 2 groups： blank group intragastrie administration by physiologic saline ,experimental group by Liuwei Dihuang Decoction. After 4,8,12,24 weeks, the NO contents of serum were examined by nitric acid reduetase, the changes of morphology of articular cartilage were observed by light microscopy and transmission electron microscopy and examined by TUNEL for apoptesis. Results： The NO activity of blank group is higher than the experimental group in any stage （ P 〈0.01 ）. Apoptosis cell index and cell proliferation index of Liuwei Dihuang Decoction group had significant differenca （ P 〈 0.01 ） . Conclusion： Liuwei Dihuang Decoction group could decrease the NO in serum of experimental osteoarthritis in rabbits, decrease the apoptosis of chondrocytes, increase chondrocytes proliferation, and maybe it＇s the mechanism of action.