小鼠低温游泳脑缺血模型的建立及在注射液量效关系上的应用

A New Model of Brain Ischemic Model in Mice by Swimming in Cold Water And Application on Injections

目的：建立一种新的脑缺血小鼠模型，用标准的注射液验证模型的可靠性。方法：小鼠分成3组：生理盐水组，尼莫地平组（1．0mg／kg）和三七总皂苷组（120mg／kg）。静脉注射25min后，动物负重5％体重，在10～10．5气的水中游泳5min，取出，断头，记录断头的喘气持续时间。小鼠分别注射尼莫地平0．3、0．7、1．0、1．5mg／kg或三七总皂苷60、80、120、160mg／kg，同上法操作，探讨两种药物的量效关系。结果：和生理盐水组比较，两个药物能显著延长小鼠断头的喘气持续时间（P〈0.01）。尼莫地平的量效关系方程式为：Y=27．74919x＋18．79446；三七总皂苷的量效关系方程式为：Y=0．13347x＋20．51017，二者的量效关系均显著（P〈0．05，P〈0．01）。结论：新的方法能够用于评价药物的抗脑缺血作用。

Objective：To evaluate effect of a new model on brain ischemia of mice. Methods： Mice were divided into three groups ： nimodipine group （1.0mg,/kg） ,physiologic saline（PS） group, and Panaxmoto Ginseng Sanponin（pns） group（ 120mg/kg）. After 25minutes of injection by vein, animals were set in a cold pool in 10 ～10.5℃for 5 minutes, loading 5% body weight. Then their heads were cut off and panting time was recorded. Mice were injected PNS in 60mg,/kg ,80mg,/kg, 120mg/kg,and 160mg/kg or nimodipine 0.3mg,/kg,0.7mg,/kg, 1.0mg,/kg,and 1.5mg,/kg respectively by vein. Both regression equations were studied in the model that the dose was independent variable and panting time was variance. Results： Comparing with PS group, the panting time of two groups was more lasted significantly （P 〈0.01 ）. The regression equation of PNS is y =0. 13347x ＋20.51017 and the regression equation of nimodipine is y = 27.74919x ＋ 18. 79446. The two dose - effect relationship in drugs appear significant linear （ P 〈 0.05 ,P 〈 0.01 ）.. Conclusion ： The new model can be applied to evaluating drug effect of anti - brain ischemia.