美洲黑杨种质资源遗传多样性的SSR分析

Analysis of Genetic Diversity of Populus deltoides Germplasm by SSR

利用12对SSR引物对美洲黑杨种质资源库的11个半同胞家系的137个子代进行遗传分析，共检测到了103个等位基因，平均每对引物检测到的等位基因数（A）为8．67，平均有效等位基因数（NP）为5．37，观测杂合度平均值（Ho）为0．39，平均期望杂合度（He）为0．75（其中10对引物的期望杂合度均大于0．65），基因分化系数（Gst）平均值为0．22，基因多样度（h）为0．55～0．72。利用UPGMA方法进行聚类分析的结果表明，家系间的遗传相似性与其地理位置差异基本相符。

One hundred and thirty seven progenies representing 11 half-sib families in Populus deltoides germplasm garden were analyzed by using twelve SSR primers. One hundred and three alleles were identified,which 8. 67 alleles per pair primers（A）. Average number of effective alleles （Ne） was 5.37. Average observed heterozygosity （Ho） was 0. 39 and average expected heterozygosity （He） was 0. 75 ,of which 10 pair primers were over 0. 65. Coefficient of gene differentiation （Gst） was 0. 22. Gene diversity（h） of families was from 0.55 to 0. 72. The result of cluster analysis by using UPGMA method indicated that identities within families accord with geographic locations.