银杏叶片蛋白质含量动态变化的电泳分析

Studies on Changes of Proteins in the Leaves of Ginkgo biloba by SDS-PAGE and IFE-SDS-PAGE

利用单向电泳和双向电泳技术,以及考马斯亮蓝染色法研究了银杏雌、雄株叶片中的营养贮藏蛋白质组分和不同季节叶片的可溶性蛋白质含量。结果表明:银杏叶片中可溶性蛋白质含量在生长旺季较高,10月下旬开始下降,至11月中旬降至最低,表明叶片脱落时养分发生了转移。电泳图谱的分析发现,随叶片可溶性蛋白质含量的变化,银杏叶片中的几种蛋白质发生变化。单向电泳结果表明,55 kD蛋白在银杏雌株中表现为11月初消失,在雄株中表现为11月中旬消失,32 kD蛋白在雌雄株中都表现为11月后消失;而双向电泳的研究除证实55 kD蛋白、32 kD蛋白发生类似变化,还发现18 kD蛋白也发生变化,即11月中旬在雌株中发生明显减少现象,在雄株中明显减少则出现在11月初。

The techniques of SDS-PAGE, IFE-SDS-PAGE, and Brilliant Blue and Commassie Dye were used to study the fractions of Vegetative Storage Proteins （VSPs） and the concentration of the soluble protein in the leaves of Ginkgo biloba. The results showed that the con-centration of the soluble protein in the leaves of Ginkgo biloba （both from a male tree and a female tree） was higher during the growing period in one year. But it began to decrease at the last ten-day of October and decreased to the lowest level at the middle ten-day of November, reflecting nitrogen nutrient transfer from the leaves to the other tissues of a tree when the leaves began to fall down. Based on the SDS-PAGE bands,it was found that VSP 55 kD band still could be clearly observed in the lane of the middle of October. While this VSP band was undetectable in the lane of the beginning of November for a female tree,and in the middle of November for a male tree. The case of VSP 32 kD was very similar to VSP 55 kD. Only difference was that VSP 32 kD could not be detected until the beginning of November for a female tree or for a male tree. With the technique of IFE-SDS-PAGE, the seasonal variations of VSPS 55 kD and 32 kD were confirmed,and also it was found that another VSP, 18 kD,sharply decreased in the middle of November for a female tree and decreased in the beginning of November for a male tree.