牛白蛋白包裹复合磁性微球的制备及性能表征

Preparation and Capability of BSA Enwrapped Composite Magnetic Microspheres

首先在反相(W/O)微乳液体系中制备出Fe3O4/PAM(聚丙烯酰胺)磁性微球;然后在牛白蛋白的磷酸氢二钠-柠檬酸缓冲溶液中,以戊二醛为交联剂,加入Fe3O4/PAM微球,使蛋白质特异性吸附在微球上。采用电子衍射、透射电镜、差热、热重、红外光谱等方法对复合微球的粒径、结构等性质进行了表征与分析。并考察了牛白蛋白的浓度、pH以及保温时间对蛋白质吸附程度的影响。结果显示,微球粒径20 nm左右,粒径均匀;微球具有较大吸附量;pH增大使蛋白质的吸附量减小;在一定范围内,增大蛋白质初始浓度和延长保温时间均有利于增加蛋白质的吸附量。

Firstly ,ferrite/polyacrylamide magnetic microspheres were prepared in inverse microemulsion system. Then, in the bovine serum albumin （BSA） buffer solution containing disodium hydrogen phosphate and citric acid, ferrite/polyacrylamide was added with crosslinker glutaraldehyde and then be enwrapped by BSA. The average particle size and structure were characterized and analyzed by ED, TEM, DSC,TG and IR. The extent of BSA adsorption was found to depend on the BSA concentration, incubation time and size, and have good the pH. The result demonstrated that the microspheres are uniform, about 20 nm in adsorption capability. Adsorption of protein diminished with the increase of pH. Increasing incubation time and BSA concentration were favorable to the adsorption of BSA.