薄皮甜瓜自交系高效组织培养技术的研究

Study on Efficacious Tissue Culture Techniques of Thin-skin Muskmelon Inbred Line

以薄皮甜瓜自交系为试材．探讨了不同部位外植体、苗龄、激素组合等因素对植株离体再生的影响。其结果是以4～6日龄的无菌苗的子叶柄、下胚轴上端外植体适于不定芽的较高频率分化；两种外植体分化存在明显极性现象，近生长点处为感受外源激素的敏感部位；MS、6-BA 1．5～2．5mg／L、ZT 1．0～3．0mg／L、NAA 0．1～0．2mg／L之间配比适于不定芽的分化，分化率达60％～70％；MS＋KT 1．5～2．5mg／L＋GA 2．0mg／L利于丛生芽的伸长生长；高效生根培养基为MS＋IBA 1．0mg／L。

The thin-skin muskmelon inbred lines were used to study the effects of explants,seedling age,and hormones on plant in vitro regeneration. The results showed that the cotyledon petiole and hypocotyls of 4～6 days seedlings had higher differentiation frequency of adventitious buds. And polarity occurred obviously during explants differentiation,the sensitive regions were near the growing point. The MS media with 6-BA 1.5～2.5 mg/L,ZT 1.0～3.0 mg/L,NAA 0. 1～0.2 mg/L was suit- able for differentiation of adventitious buds, with the differentiation rate 60%～70%. And the better enlongation media was MS ＋ KT 1.5 ～ 2.5 mg/L ＋ GA 2.0 mg/L while the high efficiency rooting media was MS＋IBA 1.0 mg/Lo