泽兰尾孢菌的产毒培养与毒素提取

Studies on the Culture of A Cercospora eupatorii Clone for Toxin Production and Toxin Extraction

对不同培养基质、培养时间、培养初始pH值、振荡培养、光照等进行研究,探索泽兰尾孢菌的产毒条件.结果表明:泽兰尾孢菌的最佳产毒条件是在PD培养液中调节初始pH值为中性、采用自然昼夜交替的光照条件全振荡发酵8 d.通过5种不同极性的有机溶剂提取毒素物质,并进行生物检测,结果显示:毒素易溶于大极性有机溶剂,且甲醇能够充分提取毒素.

The phytotoxin producing conditions of Cercospora eupatorii were experimented with different media, culture durations, initial pH values of the media, shaking intensity and illumination conditions. The results showed that the optimal cultural conditions for producing phytotoxin were in PD medium with initial pH 7. 0, natural day - night illumination alternation and with shaking culture for 8 d. 5 types of solvents with different polarities were tested for the phytotoxin extraction and then followed by bioassay, it was showed that the solvents with greater polarities, such as methyl could fully extract the toxins produced by Cercospora eupatorii.