庆大霉素雾化吸入对重型颅脑损伤大鼠AM吞噬功能的影响

Influence of inhaling gentamycin via aerosol on the phagocytosis function of alveolar macrophage in lungs of rats with severe head injury

目的研究庆大霉素雾化吸入对重型颅脑损伤(severeheadinjury,SHI)大鼠肺泡巨噬细胞(alveolarmacrophage,AM)吞噬功能的影响。方法采用大鼠颅脑局部气压冲击伤模型,将40只雄性SD大鼠随机分为正常组、对照组和实验组。正常组不予任何处理,观察24h处死动物。对照组动物创伤后给予单纯生理盐水雾化吸入。实验组动物创伤后给予生理盐水+庆大霉素4万U雾化吸入。分别于1、3、7d处死动物,用支气管肺泡灌洗获取标本。用白色念珠菌法观察AM的吞噬率。结果实验组AM吞噬率为(0.415±0.089),在7d时明显低于对照组(0.560±0.075),P<0.001。结论庆大霉素长期雾化吸入可降低AM吞噬率。

Objective To study the influence of inhaling gentamycin via aerosol on the phagocytosis function of AM in the lungs of rats with severe head injury. Methods The model of severe head injury （SHI） was established by local impact injury. 40 male SD rats were divided randomly into normal group （NG, n=4）, control group （CG, n= 18） and experimental group （EG, n= 18）. Patients in the control group inhaled normal saline via aerosol, and in the experimental group inhaled gentamycin via aerosol after injury. The phagocytosis function of AM was investigated by Oidium albicans. Results The phagocytosis percentage of EG（0. 415 ± 0. 089） were markedly lower than that of CG （0. 560±0. 075） on day 7, P 〈 0. 001. Conclusions Long-term administration of gentamycin via aerosol can obviously reduce the phagocytosis percentage of AM in the lungs of rats with severe head injury.