脊髓内源性物质对脊髓神经元在体外存活的影响

EFFECT OF THE ENDOGENOUS SPINAL CORD SUBSTANCES ON THE SURVIVAL OF THE NEURONS OF SPINAL CORD IN VITRO

神经元在体外的存活是衡量一种营养因子有无神经营养作用的重要指标之一。我们用人胚制备脊髓提取液,并用Centricon(Millipo-re)将粗提取液分成<10KD、10-30KD及>30KD三种组份,研究了粗提取液及这三种组份对体外培养中的脊髓神经元存活的影响,结果表明加粗提取液及<10KD的实验组比对照组活性要好,表现在线粒体中琥珀酸脱氢酶活性高(MTT法),神经元中NSE活性高(NSE-ELISA法)及细胞生长合成的总蛋白的量高等方面。但以<10KD组份对细胞的促活作用最强,与对照组相比有显著性差异。以上结果显示人胚脊髓中存在对脊髓神经元有促进存活的物质。

The smrvival of neurons in vitro is an important indcx to evalutate thc trophic effect of neurotrophic factors (NTFs). Crude cxtract was prepared from human fetal spinal cord and further divided into three fractions, <10 KD, 10-30 KD and >30KD with ccntricon (Milli-pore.). The effccts of thc crude cxtract and the three fractions on the survival of spinal cord neurons werc cxamined. MTT, NSE-ELISA and assay of total proteins were used to evaluate the viabi!ity of the cells. All three methods demonstrated increase viability in cultures with the crude extract and<10 KD group. It is concluded that there are neuron survival prompting substance(s) in the exttact of human fetal spinal cord.