摘要
用ＰＣＲ分析ＨＣＶ－ＲＮＡＮＳ５部分序列变异魏来，王宇，陈红松，陶其敏（北京医科大学人民医院肝病研究所，北京１０００４４）对丙型肝炎病毒（ＨＣＶ）ｃＤＮＡ的序列分析是验证ＨＣＶ基因分型，确定新的型与亚型的基础￣［１］．经典的序列分析方法需经繁琐的分子...
HCV NS5b cDNA from two HCV infected individuals were sequenced with polymerase-chain-reation (PCR)-directed sequencing method. Compared with HCV-J, 91. 84% and 92. 36 % nucleotides homology, 91. 84% and 95. 79% homology with HC-C2,were found in HC114 and HC-C11 respectively. A three nucleotide deletion occurred in HC-114. As the generated profiles of sequencing ladders were clear, it indicated that PCR-directed sequencing method worked best in showing larger range variations as well as individual base variation.
基金
美国中华医学基金
