外源性IL-18基因对C6胶质瘤细胞增殖及相关基因表达的影响

Effects of exogenous IL-18 gene on the proliferation and related gene expression in C6 glioma cells

目的:研究IL-18基因转染对C6胶质瘤细胞增殖活性及相关基因表达的影响.方法:用流式细胞仪观察C6/IL-18细胞周期、增殖指数的变化;用RT-PCR,蛋白印迹、免疫细胞化学方法分析C6/IL-18细胞cyc lin D1,cyc lin B1,Bc l-2 mR-NA及蛋白的表达.结果:与亲代C6细胞相比,C6/IL-18细胞表现为G0/G1期细胞增多、G2/M期细胞减少,细胞增殖指数(PI)降低.C6/IL-18细胞的cyc lin D1和cyc lin B1,Bc l-2 mR-NA及蛋白表达降低.结论:外源性IL-18基因可降低C6细胞的增殖活性,其机制可能与Bcl-2,cyclin B1和cyclin D1表达下调有关.

AIM： To investigate the effects of exogenous IL-18 gene on the proliferative ability and related gene expression in transfected C6 glioma cells. METHODS ： The cell cycle distributions and proliferation index of C6/IL-18 and its parental C6 cells were detected by flow cytometry. RT-PCR, immunocytochemistry and Western blot analysis were applied to test the mRNA and protein expression of cyclin D1, cyclin B1 and Bcl-2 in C6/IL-18 and C6 cells respectively. RESULTS： Compared with C6 cells, C6/IL-18 cells were arrested at G0/G1 phase with fewer G2/M cells. The proliferation index of C6/IL-18 cells was lower than that of C6 cells. At both mRNA and protein levels, the Bcl-2, cyclin B1 and cyclin D1 expression in C6/IL-18 cells were decreased. CONCLUSION： Exogenous IL-18 gene could reduce the proliferative ability of C6 cells by inhibiting the progress of cell cycle and down-regulating the Bcl-2, cyclin B1 and cyclin D1 expressions.