成都地区自身免疫型糖尿病患者临床特征及CD38基因多态性研究

Clinical Features and CD38 Polymorphisms of Latent Autoimmune Diabetes of Adults(LADA) in Chengdu

目的了解成都地区成人迟发自身免疫型糖尿病（LADA）患者的临床特征，CD38基因在内切酶PvU Ⅱ位点多态性及Arg^140Trp突变频率。方法对44例LADA、67例2型糖尿病（T2DM）患者和25例正常人（NC）的临床生化指标、胰岛自身抗体、血浆C肽水平及CD38各基因型、等位基因进行比较。结果LADA的高发年龄段在30～50岁，存在体重肥胖及超重者。LADA和T2DM存在CD38基因B／B、A／B多态，NC组无多态存在，均为B／B。三组中都未发现CD38Arg^140Trp突变。结论本研究尚未发现LADA的发病与CD38基因的多态性和CD38Arg^140Trp突变有关。

Objective To find out the clinical characteristic elements as parameters and detect the polymorphism at PvU Ⅱ endonuclease site and Arg^140Trp mutation of the CD38 gene of LADA patients in the Chengdu area. Methods The clinical parameters, islet cell-specific autoantibodies, C-peptide levels, genetic and mutation frequency of the CD38 gene in 44 patients with LADA and 67 patients with T2DM were investigated and compared with those in 25 normal controls. Results The patients of 30-50 years of age had higher prevalence of LADA. A number of LADA patients were obese or over-obese. The positivity of GAD-Ab, ICA and IAA declined with the increase of age. Both LADA and T2DM patients had the polymorphisms of B/B and A/B of the CD38 gene, but the healthy controls did not have such polymorphisms. Arg^140Trp mutation was not found in both patients and healthy controls. Conclusion This research revealed no relationship between the on-set of LADA and the CD38 polymorphism and CD38 Arg^140Trp mutation.