肥胖儿童食欲素、瘦素和神经肽Y水平及其相互关系

Blood Leptin,Orexins and NPY Levels and Their Relations in Obese Children

目的分析肥胖儿童血中食欲素(O rex ins)、瘦素(L eptin、LEP)和神经肽Y(neuropeptide Y,NPY)水平及其相互关系,探讨O rex ins,LEP和NPY在儿童肥胖发生中的作用及三者存在的相互作用关系。方法以放射免疫法测定98名肥胖〔BM I男(29.24±1.87)kg/m2,女(28.12±2.30)kg/m2〕和104名正常对照〔BM I男(20.49±1.95)kg/m2,女(19.59±1.51)kg/m2〕儿童血中的LEP、O rex ins和NPY浓度。结果肥胖儿童血中LEP浓度〔男(26.00±14.66)ng/mL;女(33.59±14.63)ng/mL〕高于对照组儿童〔男(6.65±4.49)ng/mL;女(10.48±5.52)ng/mL,P<0.01〕;肥胖儿童血中O rex inA浓度〔男(3.23±1.86)pg/mL;女(3.38±1.80)pg/mL〕低于对照组〔男(4.52±1.52)pg/mL;女(4.71±1.53)pg/mL,P<0.05〕;两组间LEP与NPY皆呈负相关关系(对照组r=-0.310;肥胖组r=-0.302,P均<0.01),但斜率不同(对照组-2.969;肥胖组-0.809);NPY与O rex inA为正相关关系r=0.207 P<0.05,肥胖组儿童相对于对照组O rex inA的波动范围明显变小。结论肥胖儿童与对照组相比血中LEP与O rex inA浓度水平相反,儿童肥胖的发生与LEP、O rex inA、NPY和它们之间的相互作用有关。

Objective To investigate the concentration levels of leptin, orexins and neuropeptide Y （NPY） in the blood of obese children, and to analysed the relationship between these substances. Methods RIA methods were used to measure the concentrations of leptin, orexinA, orexinB, and NPY in the blood of 98 obese children [BMI： male （29.24±1.87） kg/m^2, female （28.12±2.30） kg/m^2] and in 104 normal children [BMI： male （20.49±1.95） kg/m^2, female （19.59±1.51） kg/m^2] as the control group. Results The leptin concentrations in obese children [male （26.00±14.66） ng/mL; female （33.59±14.63） ng/mL] were higher than those in the control group [male （6.65±4.49） ng/mL; female [10.48±5.52） ng/mL P〈0.01]. The concentrations of plasma orexinA in obes children [male （3.23±1.86） pg/mL; female （3.38±1.80） pg/mL] were lower than those in the control group [male （4.52±1.52） pg/mL; female （4.71±1.53） pg/mL P〈0.05]; Negative correlations between leptin and NPY were noted in the obesity group （r=-0.302） and the control group（r=-0.310, P〈0.01）, while the slopes in the two groups were different （control group -2.969; obese group -0.809）. A positive correlation between NPY and orexinA was noted （r = 0.207. P〈0.05）. The fluctuation range of orexinA in obese children was markedly narrowed when compared with that in the control group. Conclusion The concentration level of peripheral orexinA and leptin in the obese and non-obese children change inversely. The obesity in children correlates with the concentrations of orexinA, leptin. NPY as well as with their interactions.