摘要
目的:探讨胃癌组织中类肝素酶、VEGF-D表达及其与胃癌临床病理参数及血管、淋巴管生成的关系。方法:采用逆转录聚合酶链反应(RT-PCR)法及免疫组织化学方法分别检测48例胃癌及癌旁组织中类肝素酶mRNA和VEGF-D的表达。结果:类肝素酶在胃癌组织中的阳性表达显著高于癌旁组织(P<0.05)。在高中分化、Ⅰ~Ⅱ期的胃癌组织中类肝素酶的表达明显比低分化、Ⅲ~Ⅳ期减低(P<0.01)。有淋巴结转移组类肝素酶表达明显比无淋巴结转移组增高(P<0.01)。VEGF-D在胃癌组织中表达亦显著高于癌旁组织(P<0.05),且亦与淋巴结转移有关(P<0.05)。在类肝素酶阳性表达组VEGF-D表达显著高于类肝素酶阴性表达组(P<0.05)。结论:类肝素酶促进了胃癌的生长、浸润及转移和血管、淋巴管生成,可作为胃癌预后的参考指标。
Objective: To determine the expression levels of heparanase mRNA and VEGF-D in gastric cancer and to describe a correlation between these expression levels and the clinicopathologic parameters, angiogenesis and lymphangiogenesis. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to detect heparanase mRNA levels and immunohistochemical staining was used to detect VEGF-D in the cancerous and paraneoplastic tissues of 48 cases with gastric carcinoma. Results: The expression of heparanase was significantly higher in gastric cancer tissues than in paraneoplastic tissues (P〈0.05). The positive rate of heparanase expression in well- and moderately differentiated cancer (stage Ⅰ to Ⅱ ) was significantly lower than that in poorly differentiated cancer (stage Ⅲ to Ⅳ (〈0.05)). In gastric cancer with lymph node metastasis, heparanase expression was significantly higher than that in gastric cancer without lymph metastasis (P〈0.05). The expression of VEGF-D was also significantly higher in gastric cancer tissues than in cancer-adjacent tissues (P〈0.05). The positive rate of VEGF-D expression was correlated with lymph-node metastasis (P〈0.05).The expression of VEGF-D in the group with positive expression of heparanase was higher than that in the group with negative expression of heparanase (P〈0.05). Conclusion: Heparanase can facilitate the infiltration, lymph-node metastasis and microvessel formation of gastric cancer and may also be a useful marker for predicting the prognosis of patients with gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第13期731-733,共3页
Chinese Journal of Clinical Oncology
基金
安徽省教育厅自然科学研究基金资助(编号:2003kj317)