摘要
目的:探讨胃癌组织中类肝素酶、VEGF-D表达及其与胃癌临床病理参数及血管、淋巴管生成的关系。方法:采用逆转录聚合酶链反应(RT-PCR)法及免疫组织化学方法分别检测48例胃癌及癌旁组织中类肝素酶mRNA和VEGF-D的表达。结果:类肝素酶在胃癌组织中的阳性表达显著高于癌旁组织(P<0.05)。在高中分化、Ⅰ～Ⅱ期的胃癌组织中类肝素酶的表达明显比低分化、Ⅲ～Ⅳ期减低(P<0.01)。有淋巴结转移组类肝素酶表达明显比无淋巴结转移组增高(P<0.01)。VEGF-D在胃癌组织中表达亦显著高于癌旁组织(P<0.05),且亦与淋巴结转移有关(P<0.05)。在类肝素酶阳性表达组VEGF-D表达显著高于类肝素酶阴性表达组(P<0.05)。结论:类肝素酶促进了胃癌的生长、浸润及转移和血管、淋巴管生成,可作为胃癌预后的参考指标。
Objective： To determine the expression levels of heparanase mRNA and VEGF-D in gastric cancer and to describe a correlation between these expression levels and the clinicopathologic parameters, angiogenesis and lymphangiogenesis. Methods： Reverse transcription polymerase chain reaction （RT-PCR） was used to detect heparanase mRNA levels and immunohistochemical staining was used to detect VEGF-D in the cancerous and paraneoplastic tissues of 48 cases with gastric carcinoma. Results： The expression of heparanase was significantly higher in gastric cancer tissues than in paraneoplastic tissues （P〈0.05）. The positive rate of heparanase expression in well- and moderately differentiated cancer （stage Ⅰ to Ⅱ ） was significantly lower than that in poorly differentiated cancer （stage Ⅲ to Ⅳ （〈0.05））. In gastric cancer with lymph node metastasis, heparanase expression was significantly higher than that in gastric cancer without lymph metastasis （P〈0.05）. The expression of VEGF-D was also significantly higher in gastric cancer tissues than in cancer-adjacent tissues （P〈0.05）. The positive rate of VEGF-D expression was correlated with lymph-node metastasis （P〈0.05）.The expression of VEGF-D in the group with positive expression of heparanase was higher than that in the group with negative expression of heparanase （P〈0.05）. Conclusion： Heparanase can facilitate the infiltration, lymph-node metastasis and microvessel formation of gastric cancer and may also be a useful marker for predicting the prognosis of patients with gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第13期731-733,共3页
Chinese Journal of Clinical Oncology
基金
安徽省教育厅自然科学研究基金资助(编号:2003kj317)
