转基因苹果组培苗铁蛋白基因在转录水平上的表达

Study on the expression of ferritin gene transcript from transgenic apple plants

以转菜豆铁蛋白基因的嘎拉苹果4个株系的试管苗为材料,通过定量RT-PCR的方法检测外源和内源铁蛋白基因的转录表达量,结果表明苹果转基因植株外源铁蛋白基因的转录表达量与其插入的拷贝数没有明显的关系,可能受插入位点的影响;同时在铁诱导的条件下,外源铁蛋白基因与内源的铁蛋白基因的转录表达特性一致,转录水平受铁的调控,随着铁诱导时间的延长,其mRNA表达水平逐渐增加。对转基因植株的根、茎、叶3个部位铁蛋白基因的转录表达量研究结果表明,外源菜豆铁蛋白基因与内源苹果铁蛋白基因的mRNA含量均在根部最高,茎次之,叶片最低。

The expression level of ferritin mRNA of transgenic apple plants with recombinant bean iron-binding gene was analyzed by real time quantitative RT-PCR. The results indicated that exogenous ferritin mRNA abundance had no correction with the copies in the genome of Malus domestica Borkh., possibly affected by inert position. Also the results of real time quantitative RT-PCR analysis confirmed exogenous ferritin mRNA transcript in response to iron was similar with endogenous mRNA ferritin, and expression of the ferritin transcript of seedlings increased gradually with induced time after treatment with 0.5 mmol/L iron-citrate; the results indicated that the expression of exogenous ferritin gene was regulated in response to iron at transcriptional level, The results of real time quantitative RT-PCR analysis demonstrated both exogenous ferritin gene and endogenous ferritin gene mRNA accumulation are highest in roots, higher in stems, less in leaves.