摘要
以多个野生桃秋季成熟叶片为材料,采用改进CTAB法对其基因组总DNA的提取进行了研究,并与其相应幼叶DNA的提取效果进行了比较分析。结果表明,从野生桃成熟叶片提取的DNA除产量低于幼叶外,DNA的纯度和完整性都较好,D260nm/D280nm的比值均为1.8～2.0,无降解现象,RNA去除干净,能被限制性内切酶完全消化;其AFLP分析(引物EcoRI-TA/MseI-CTT)条带清晰,多态性好,说明此方法提取的野生桃成熟叶片基因组总DNA完全适于AFLP分析。
The genomic DNA samples of mature leaves of several wild peach varieties in autumn were isolated by improved CTAB method ,and DNA extraction effects were contrasted with their tender leaves. The results showed： the extracted DNA of mature leaves by this method was pure,integral,the value of D360nm/D280nm was 1.80 to 2.0,degradation was nearly free,the RNA was eliminated completely, and suitable for digestion by restriction endonucleases, except the yields of DNA extraction from mature leaves was lower than that of the tender leaves. AFLP analysis indicated the primer （EcoRI-TA/MseI-CTT） produced clear polymorphic patterns. Therefore this method could be used for extracting ideal DNA samples from mature leaves of wild peach and suitable for AFLP.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第4期638-641,共4页
Journal of Fruit Science
基金
北京市自然科学基金资助项目(5042007)
