verteporfin光动力疗法对体外培养的成人视网膜色素上皮细胞色素上皮衍生因子、血管内皮生长因子表达水平的影响

Influence of photodynamic therapy on expression of pigment epithelial derivative factor and vascular endothelial growth factor in adult human retinal pigment epithelial cells

目的观察经光敏剂verteporfin光动力疗法(PDT)处理后的体外培养的成人视网膜色素上皮(RPE)细胞色素上皮衍生因子(PEDF)、血管内皮生长因子(VEGF)mRNA表达水平的改变。方法采用噻唑蓝比色法(MTT)测定PDT前后体外培养的成人RPE细胞活性的变化。应用半定量逆转录聚合酶链式反应(RT-PCR)测定PDT前后RPE细胞PEDF和VEGFmRNA表达水平的变化。结果PDT可造成RPE细胞死亡,死亡率与激光能量密度和光敏剂浓度成正相关。PDT后RPE细胞表达PEDF、VEGFmRNA水平下降,下降程度与激光能量密度和光敏剂浓度成正相关。结论PDT可下调体外培养的成人RPE细胞PEDF、VEGFmRNA的表达水平,这可能与PDT治疗黄斑下脉络膜新生血管膜(CNVM)后CNVM的消退或再生有关。

Objective To investigate the impact of photodynamic therapy （PDT） with verteporfin on the expression of pigment epithelial derivative factor （PEDF） mRNA and vascular endothelial growth factor （VEGF） mRNA in adult retinal pigment epithelial （RPE） cells in vitro. Methods The changes of cellular viability before and after PDT were assessed by methyl thiazolyl tetrazolum （MTT） colorimetric assay. Semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） was conducted to detect the expression of PEDF and VEGF mRNA in RPE cells before and after PDT. Results PDT caused the death of RPE cells. The cellular mortality was positively correlated with the power of photocoagulation and the concentration of verteporfin. Conclusion PDT could down-regulate the expression of PEDF and VEGF mRNA in adult RPE cells in vitro, which may relate to the cure or relapse of subfoveal choroidal neovascular membrane after PDT.