摘要
Aquaporins 玩在植物水关系的一个重要角色。为了进一步在水下面在植物理解 aquaporin 功能，强调， aquaporins 的亚群的表示，血浆膜内在的蛋白质(果仁) ，在高地米饭在蛋白质和 mRNA 水平被学习(Oryza sativa L。cv。Zhonghan 3 ) 并且低地米饭(Oryza sativa L。cv。Xiushui 63 ) 他们什么时候是水，与 20% 聚乙烯乙二醇(木钉) 由处理强调了。植物对 20% 木钉处理不同地作出回应。高地米饭叶子的叶水内容很快被减少。果仁蛋白质水平在木钉处理的 10 h 以后在两种类型的根，但是仅仅在高地米饭的叶子显著地增加了。在 mRNA 水平， OsPIP1； 2， OsPIP1； 3， OsPIP2； 1 并且 OsPIP2； 5 在象 OsPIP1 一样的根； 2 并且 OsPIP1； 3 在高地米饭在叶子是显著地起来调整的，而相应基因仍然保持在低地米饭未改变或下面调整。同时，我们在内长的 abscisic 酸(骆驼毛的织物) 观察了重要增加在高地米饭然而并非在在水赤字下面的低地米饭的水平。有 60 microM 骆驼毛的织物的处理提高了 OsPIP1 的表示； 2， OsPIP2； 5 并且 OsPIP2； 6 在根和 OsPIP1； 2， OsPIP2； 4 并且 OsPIP2； 6 在高地米饭的叶子。到水应力和骆驼毛的织物的果仁基因的应答的海角是不同的，暗示果仁基因的规定包含在水赤字期间的骆驼毛的织物依赖者和骆驼毛的织物无关的发信号小径。
Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins （PIPs）, was studied at both the protein and mRNA level in upland rice （Oryza sativa L. cv. Zhonghan 3） and lowland rice （Oryza sativa L. cv. Xiushui 63） when they were water stressed by treatment with 20% polyethylene glycol （PEG）. Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1,2, OsPIP1,3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1,2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid （ABA） level in upland rice but not in lowland rice under water deficit. Treatment with 60 μM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling oathways during water deficit.
